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. 1977 Nov 1;167(2):419-28.
doi: 10.1042/bj1670419.

Affinity chromatography of aminoacyl-transfer ribonucleic acid synthetases. Cognate transfer ribonucleic acid as a ligand

C M Clarke, J R Knowles

Affinity chromatography of aminoacyl-transfer ribonucleic acid synthetases. Cognate transfer ribonucleic acid as a ligand

C M Clarke et al. Biochem J. .

Abstract

The use of tRNA affinity columns for the purification of aminoacyl-tRNA synthetases was investigated. A purification method for valyl-tRNA synthetase from Bacillus stearothermophilus is described that uses two affinity columns, one containing the pure cognate tRNA, and the other containing all tRNA species except the cognate tRNA. A method for the rapid preparation of the two columns was developed, which does not require prior isolation of cognate tRNA but makes use of the ability of the target synthetase to select its cognate tRNA. The usefulness of tRNA columns is compared with that of affinity columns derived from the aminoalkyladenylate reported in the preceding paper [Clarke & Knowles (1977) Biochem J. 167, 405-417].

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References

    1. J Biol Chem. 1970 Jun;245(12):3059-65 - PubMed
    1. Eur J Biochem. 1972 Dec 4;31(2):335-44 - PubMed
    1. Biochim Biophys Acta. 1973 Apr 28;304(2):231-5 - PubMed
    1. Eur J Biochem. 1973 Nov 1;39(1):265-73 - PubMed
    1. Eur J Biochem. 1974 Jun 15;45(2):351-62 - PubMed

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