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. 2011;18(1):e00008.
doi: 10.1042/CBR20110002. Epub 2011 Mar 18.

Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells

Jee Eun Han  1 , Casiano H Choresca, Ok Jae Koo, Hyun Ju Oh, So Gun Hong, Ji Hyung Kim, Sang Phil Shin, Jin Woo Jun, Byeong Chun Lee, Se Chang Park
Affiliations

Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells

Jee Eun Han et al. Cell Biol Int Rep (2010). 2011.

Abstract

Genetically manipulated transparent animals were already explored in many species for in vivo study of gene expression, transplantation analysis and cancer biology. However, there are no reports about transparent animals as in vitro genetic resources. In the present study, fin-derived cells from glass catfish (Krytopterus bicirrhis), naturally transparent fish with a visible skeleton and internal organs, were isolated after culturing fin explants and characterized using cryopreservation and cell cycle analysis. The cells grew well in DMEM (Dulbecco's modified Eagle's medium) containing 1% (v/v) P/S (penicillin-streptomycin) and 10% (v/v) fetal bovine serum at 26°C and showed increased cryopreservation efficiency with the slow-freezing method in the presence of 15% dimethyl sulfoxide. In addition, cell cycle analysis was evaluated based on flow cytometric analysis, and culturing to confluence (>85%) was more effective for synchronizing cells at the G(0)/G(1) stages than roscovitine treatment (<75%). This is the first report about cell isolation from transparent animals. The results from testing the cell's viability following cryopreservation and subjecting the cells to cycle analysis can be useful tools for genetic resource management.

Keywords: DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; P/S, penicillin–streptomycin; SCNT, somatic cell nuclear transfer; cell cryopreservation; cell cycle analysis; cell isolation; fin cells; glass catfish.

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Figures

Figure 1
Figure 1. Photomicrograph of cultured cells from glass catfish and the growth rate comparison of three fintypes (caudal, pectoral and abdominal)
(A) Monolayer formation from pectoral fin at passage seven, scale bar = 0.2 mm. (B) Scale bar = 80 μm. (C) Scale bar = 40 μm. (D) Single cell from pectoral fin at passage seven, scale bar = 40 μm. (E) Differences in growth rate among three fin types (means±S.E.M.).
Figure 2
Figure 2. Typical histograms of DNA content obtained using flow cytometry of glass catfish fin cells at (A) culturing to confluence, (B) 10 μM roscovitine treatment
See this image and copyright information in PMC

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