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Clinical Trial
. 2013 Feb;46(3):278-81.
doi: 10.1016/j.clinbiochem.2012.10.032. Epub 2012 Oct 31.

Comparison of hematologic measurements between local and central laboratories: data from the BABY HUG trial

Ram Kalpatthi  1 , Bruce Thompson, Ming Lu, Winfred C Wang, Niren Patel, Abdullah Kutlar, Thomas Howard, Lori Luchtman-Jones, Scott T Miller; BABY HUG Investigators
Collaborators, Affiliations
Clinical Trial

Comparison of hematologic measurements between local and central laboratories: data from the BABY HUG trial

Ram Kalpatthi et al. Clin Biochem. 2013 Feb.

Abstract

Objectives: To investigate the concordance of blood count indices measured locally and at a central laboratory.

Design and methods: In a multi-center clinical trial of hydroxyurea therapy in infants with sickle cell anemia (BABY HUG), the concordance between blood count indices measured locally and at a central laboratory was investigated.

Results: Local laboratory measurements of neutrophil and monocyte counts were significantly higher (44% and 37%, respectively) compared to the central measurements (p<0.0001), and mean corpuscular volume (MCV) was higher centrally.

Conclusion: Overnight shipping with processing delay causes spurious reductions in absolute neutrophil count (ANC) and absolute monocyte count (AMC) that may result in incorrect monitoring decisions in multicenter clinical trials.

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Figures

Figure 1
Figure 1
Bland-Altman plots of hematological measurements. Y-axis is the difference between local and central measurements; x-axis is the average of the local and central measurements.
See this image and copyright information in PMC

References

    1. Bayle C, Bensmaine M, Henry-Amar M. effect of time factors and transportation on circulating neutrophil counts in patients with chemotherapy-induced neutropenia. Pathol Biol (Paris) 1992;40:25–30. - PubMed
    1. Cornet E, Behier C, Troussard X. Guidance for storing blood samples in laboratories performing complete blood count with differential. Int J Lab Hematol. 2012 - PubMed
    1. Wang WC, Ware RE, Miller ST, Iyer RV, Casella JF, Minniti CP, et al. Hydroxycarbamide in very young children with sickle-cell anaemia: A multicentre, randomised, controlled trial (baby hug) Lancet. 377:1663–72. - PMC - PubMed
    1. Thompson BW, Miller ST, Rogers ZR, Rees RC, Ware RE, Waclawiw MA, et al. The pediatric hydroxyurea phase iii clinical trial (baby hug): Challenges of study design. Pediatr Blood Cancer. 2010;54:250–5. - PMC - PubMed
    1. Betensky RA, Connick E, Devers J, Landay AL, Nokta M, Plaeger S, et al. Shipment impairs lymphocyte proliferative responses to microbial antigens. Clin Diagn Lab Immunol. 2000;7:759–63. - PMC - PubMed