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. 1990 Feb;28(2):319-23.
doi: 10.1128/jcm.28.2.319-323.1990.

Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria

K Dix  1 , S M Watanabe, S McArdle, D I Lee, C Randolph, B Moncla, D E Schwartz
Affiliations

Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria

K Dix et al. J Clin Microbiol. 1990 Feb.

Abstract

Oligodeoxynucleotide probes were developed for identification of the periodontal bacteria Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, B. intermedius types I and II, B. forsythus, Eikenella corrodens, Fusobacterium nucleatum, Haemophilus aphrophilus, Streptococcus intermedius, and Wolinella recta. Probes were designed by sequencing the 16S rRNA for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. These probes were specific when tested against a panel of nucleic acids from closely related bacteria.

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