Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Dec;29(12):1327-34.
doi: 10.1007/s10815-012-9885-9. Epub 2012 Nov 8.

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

T Sivanarayana  1 , Ch Ravi Krishna, G Jaya Prakash, K Murali Krishna, K Madan, B Sireesha Rani, G Sudhakar, G A Rama Raju
Affiliations

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

T Sivanarayana et al. J Assist Reprod Genet. 2012 Dec.

Abstract

Purpose: The present study was undertaken to evaluate the effects of morphokinetic abnormalities of human spermatozoa on chromatin packing and DNA integrity and possible beneficial effects of sperm selection in ICSI.

Methods: Semen samples from 1002 patients were analysed for morphology and motility using CASA. Protamine status and DNA fragmentation were analysed by chromomycin A3 staining and sperm chromatin dispersion assay respectively.

Results: Sperms with elongated, thin, round, pyri, amorphous, micro and macro forms were significantly higher in teratozoospermic and oligoasthenoteratozoospermic groups. Significant difference in chromatin packing and DNA fragmentation index was observed in these abnormal groups compared with normal. Similarly significant correlation was also seen between abnormal motility parameters and DNA fragmentation index in asthenozoospermic group compared with normal.

Conclusions: Specific abnormal morphological forms have higher incidence of chromatin packing abnormalities and DNA fragmentation. Using these sperms in ICSI might have an impact on fertilization, embryo development and abortion rates. These can be selectively avoided during ICSI procedure to improve ART outcome.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. World Health Organization. WHO laboratory manual for the examination of human semen and semen-cervical mucus interaction. 2nd (eds), Cambridge, Cambridge Univ. Press, Cambridge, 1987.
    1. Kruger TF, Menkveld R, Stander FS, Lombard CJ, Vander Merwe JP, Vanzyl JA, Smith K. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril. 1986;46:1118–23. - PubMed
    1. Grow DR, Oehninger S, Seltman HJ, Toner JP, Swanson RJ, Kruger TF, Muasher SJ. Sperm morphology as diagnosed by strict criteria: probing the impact of teratozoospermia on fertilization rate and pregnancy outcome in a large in vitro fertilization population. Fertil Steril. 1994;62:559–67. - PubMed
    1. Morgentaler A, Fung MY, Harris DH, Powers RD, Alper MM. Sperm morphology and in vitro fertilization outcome: a direct comparison of World Health Organization and strict criteria methodologies. Fertil Steril. 1995;64:1177–82. - PubMed
    1. Jorgensen N, Auger J, Giwercman A, Irvine DS, Jensen TK, Jouannet P, Keiding N, Bon C, MacDonald E, Pekuri AM, Scheike T, Simonsen M, Suominen J, Skakkeboek NE. Semen analysis performed by different laboratory teams: an intervariation study. Int J Androl. 1997;20:201–08. doi: 10.1046/j.1365-2605.1997.00052.x. - DOI - PubMed