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. 2013 Jan;51(1):328-30.
doi: 10.1128/JCM.02225-12. Epub 2012 Nov 7.

Genetic analysis of non-hydrogen sulfide-producing Salmonella enterica serovar typhimurium and S. enterica serovar infantis isolates in Japan

Chieko Sakano  1 , Makoto Kuroda, Tsuyoshi Sekizuka, Taisei Ishioka, Yukio Morita, Akihide Ryo, Hiroyuki Tsukagoshi, Yuko Kawai, Nobuko Inoue, Hayato Takada, Yumiko Ogaswara, Atsuyoshi Nishina, Masa-aki Shimoda, Kunihisa Kozawa, Kazunori Oishi, Hirokazu Kimura
Affiliations

Genetic analysis of non-hydrogen sulfide-producing Salmonella enterica serovar typhimurium and S. enterica serovar infantis isolates in Japan

Chieko Sakano et al. J Clin Microbiol. 2013 Jan.

Abstract

Whole-genome sequencing of non-H(2)S-producing Salmonella enterica serovar Typhimurium and S. enterica serovar Infantis isolates from poultry meat revealed a nonsense mutation in the phsA thiosulfate reductase gene and carriage of a CMY-2 β-lactamase. The lack of production of H(2)S might lead to the incorrect identification of S. enterica isolates carrying antimicrobial resistance genes.

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Figures

Fig 1
Fig 1
Schematic representation of the nonsense mutation in the phsA gene encoding the thiosulfate reductase subunit. Nucleotide variations and corresponding amino acid mutations are shown below the gene organization schemes.
Fig 2
Fig 2
PFGE-Southern blot hybridization for blaCMY-2 detection. (A) S1 nuclease-PFGE analysis of the Salmonella isolates. (B) Southern blot obtained using a blaCMY-2-specific DNA probe. M, lambda ladder marker for PFGE.
See this image and copyright information in PMC

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