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. 2013 Jan;51(1):348-51.
doi: 10.1128/JCM.02510-12. Epub 2012 Nov 7.

Clinical application of a multiplex real-time PCR assay for simultaneous detection of Legionella species, Legionella pneumophila, and Legionella pneumophila serogroup 1

Alvaro J Benitez  1 , Jonas M Winchell
Affiliations

Clinical application of a multiplex real-time PCR assay for simultaneous detection of Legionella species, Legionella pneumophila, and Legionella pneumophila serogroup 1

Alvaro J Benitez et al. J Clin Microbiol. 2013 Jan.

Erratum in

  • J Clin Microbiol. 2014 Feb;52(2):709

Abstract

We developed a single-tube multiplex real-time PCR assay capable of simultaneously detecting and discriminating Legionella spp., Legionella pneumophila, and Legionella pneumophila serogroup 1 in primary specimens. Evaluation of 21 clinical specimens and 115 clinical isolates demonstrated this assay to be a rapid, high-throughput diagnostic test with 100% specificity that may aid during legionellosis outbreaks and epidemiologic investigations.

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Figures

Fig 1
Fig 1
Multiplex assay for detection of Legionella spp., Legionella pneumophila, Legionella pneumophila sg1, and human DNA. (A) L. anisa DNA/human DNA; (B) L. pneumophila sg2 DNA/human DNA; (C) L. pneumophila sg1 DNA/human DNA. Samples were run in duplicate. All channels are shown to display specificity. Delta Rn, baseline-corrected fluorescence.
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References

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