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. 2012 Jan;61(5):480-94.
doi: 10.1270/jsbbs.61.480. Epub 2012 Feb 4.

Recent advances in soybean transformation and their application to molecular breeding and genomic analysis

Tetsuya Yamada  1 , Kyoko Takagi, Masao Ishimoto
Affiliations

Recent advances in soybean transformation and their application to molecular breeding and genomic analysis

Tetsuya Yamada et al. Breed Sci. 2012 Jan.

Abstract

Herbicide-resistant transgenic soybean plants hold a leading market share in the USA and other countries, but soybean has been regarded as recalcitrant to transformation for many years. The cumulative and, at times, exponential advances in genetic manipulation have made possible further choices for soybean transformation. The most widely and routinely used transformation systems are cotyledonary node-Agrobacterium-mediated transformation and somatic embryo-particle-bombardment-mediated transformation. These ready systems enable us to improve seed qualities and agronomic characteristics by transgenic approaches. In addition, with the accumulation of soybean genomic resources, convenient or promising approaches will be requisite for the determination and use of gene function in soybean. In this article, we describe recent advances in and problems of soybean transformation, and survey the current transgenic approaches for applied and basic research in Japan.

Keywords: Agrobacterium tumefaciens; Soybean [Glycine max (L.) Merrill]; particle bombardment; transformation.

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