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. 2013:957:189-202.
doi: 10.1007/978-1-62703-191-2_13.

Studying the roles of Aurora-C kinase during meiosis in mouse oocytes

Kuo-Tai Yang  1 , Yi-Nan Lin, Shu-Kuei Li, Tang K Tang
Affiliations

Studying the roles of Aurora-C kinase during meiosis in mouse oocytes

Kuo-Tai Yang et al. Methods Mol Biol. 2013.

Abstract

We previously isolated Aurora-C (Aurkc/Aie1) in a screen for kinases expressed in mouse sperm and eggs. Aurora-C kinase was reported to be a chromosomal passenger protein that plays critical roles in chromosome alignment, segregation, kinetochore-microtubule attachment, and cytokinesis in female mouse meiosis. This chapter describes experimental approaches for examining the subcellular localization and function of Aurora-C kinase during female mouse meiosis, presenting detailed methods for introducing exogenous Aurora-C wild-type and kinase-dead mutant mRNAs into mouse oocytes by cytosolic microinjection, and preparing whole-mount meiotic oocytes and chromosome spreads for confocal immunofluorescence microscopy.

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