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. 2012 Dec 7;51(50):12529-33.
doi: 10.1002/anie.201207771. Epub 2012 Nov 8.

Novel activity-based probes for broad-spectrum profiling of retaining β-exoglucosidases in situ and in vivo

Wouter W Kallemeijn  1 , Kah-Yee Li, Martin D Witte, André R A Marques, Jan Aten, Saskia Scheij, Jianbing Jiang, Lianne I Willems, Tineke M Voorn-Brouwer, Cindy P A A van Roomen, Roelof Ottenhoff, Rolf G Boot, Hans van den Elst, Marthe T C Walvoort, Bogdan I Florea, Jeroen D C Codée, Gijsbert A van der Marel, Johannes M F G Aerts, Herman S Overkleeft
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Novel activity-based probes for broad-spectrum profiling of retaining β-exoglucosidases in situ and in vivo

Wouter W Kallemeijn et al. Angew Chem Int Ed Engl. .

Abstract

A high-end label: Cyclophellitol aziridine-type activity-based probes allow for ultra-sensitive visualization of mammalian β-glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non-mammalian β-glucosidases (see picture). These probes offer new ways to study β-exoglucosidases, and configurational isomers of the cyclophellitol aziridine core may give activity-based probes targeting other retaining glycosidase families.

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