NG-nitro-L-arginine (N5-[imino(nitroamino)methyl]-L-ornithine) impairs endothelium-dependent dilations by inhibiting cytosolic nitric oxide synthesis from L-arginine

Abstract

We studied the effects of the L-arginine analogue NG-nitro-arginine (L-NNA), in comparison with its D-isomer [D-NNA), on endothelium-dependent dilations of rabbit femoral arteries (RFA) and on the release of endothelium-derived relaxant factor (EDRF) from native and cultured endothelial cells. In addition, we examined the effects of L- and D-NNA on the L-arginine- and NADPH-dependent synthesis of nitric oxide (NO) in the cytosol of porcine aortic endothelial cells. L-NNA enhanced the noradrenaline-induced contraction of endothelium-intact, but not of endothelium-denuded segments of RFA, indicating an inhibition of basal EDRF release. L-NNA also inhibited significantly the endothelium-dependent dilations to acetylcholine (ACh). Both effects of L-NNA were attenuated by L-arginine. L-NNA rapidly inhibited the release of EDRF from cultured and native endothelial cells stimulated with thimerosal or ACh. L-NNA concentration-dependently and reversibly antagonized the L-arginine- and NADPH-dependent activation of a purified soluble guanylate cyclase (GC) by cytosol from freshly harvested porcine aortic endothelial cells, suggesting a direct competition between L-NNA and L-arginine at the level of endothelial NO-synthesis. D-NNA was ineffective in all instances. These results prove L-NNA to be a stereospecific inhibitor of the cytosolic NO formation from L-arginine in endothelial cells. Therefore, L-NNA will be a useful tool to elucidate the molecular mechanism of mammalian NO synthesis.