Pentoxifylline-induced modulation of human leukocyte function in vitro
- PMID: 2316627
- PMCID: PMC1877473
Pentoxifylline-induced modulation of human leukocyte function in vitro
Abstract
We previously demonstrated that pentoxifylline stimulated leukocyte migration in vitro and leukocyte accumulation in vivo and protects neonatal mice from experimentally induced Staphylococcus aureus infections. In the present studies we have investigated pentoxifylline's effect on human leukocyte function in vitro. In these studies we demonstrate that pentoxifylline at low concentrations (ie, 0.01 and 0.1 mg/ml) stimulates both leukocyte migration and microbicidal activity in vitro. Alternatively, low concentrations (0.001 to 0.1 mg/ml) of pentoxifylline had no significant effect on the binding uptake of S. aureus by leukocytes, nor did it enhance phagocytic degranulation. At extremely low concentrations (0.001 mg/ml), pentoxifylline enhanced oxygen metabolism by human leukocytes, as reflected by increased H2O2 production and chemiluminescence (CL). At higher concentrations (ie, 0.1 to 1 mg/ml), pentoxifylline consistently suppressed these leukocyte functions in vitro. Thus, this study supports the following hypothesis: 1) the in vivo effects of pentoxifylline may involve a direct effect on both leukocyte mobilization and microbicidal activity, and 2) the enhanced microbicidal activity induced by pentoxifylline may be a result of enhanced leukocyte oxygen metabolism. In summary, pentoxifylline appears to be an interesting immunomodulator (ie, immunoenhancement and immunosuppression) of leukocyte function in vitro, but additional studies will be required before the efficacy of pentoxifylline in man can be determined.
Similar articles
-
Pentoxifylline enhancement of defective neutrophil function and host defense in neonatal mice.Am J Pathol. 1987 Nov;129(2):217-22. Am J Pathol. 1987. PMID: 3674202 Free PMC article.
-
Effects of pentoxifylline administration on blood viscosity and leukocyte cytoskeletal function in patients with intermittent claudication.J Lab Clin Med. 1990 Jun;115(6):738-44. J Lab Clin Med. 1990. PMID: 2366034
-
Effect of pentoxifylline on polarization and migration of human leukocytes.J Leukoc Biol. 2002 Apr;71(4):588-96. J Leukoc Biol. 2002. PMID: 11927644
-
[New aspects of the pharmacology of pentoxifylline].J Mal Vasc. 1989;14 Suppl A:35-41. J Mal Vasc. 1989. PMID: 2654314 Review. French.
-
Cytokines, phagocytes, and pentoxifylline.J Cardiovasc Pharmacol. 1995;25 Suppl 2:S20-2. doi: 10.1097/00005344-199500252-00005. J Cardiovasc Pharmacol. 1995. PMID: 8699856 Review.
Cited by
-
Mechanism of pentoxifylline mediated down-regulation of killer lineage cell functions.Mediators Inflamm. 1993;2(5):379-84. doi: 10.1155/S0962935193000535. Mediators Inflamm. 1993. PMID: 18475549 Free PMC article.
-
Pattern of cytokines and pharmacomodulation in sepsis induced by cecal ligation and puncture compared with that induced by endotoxin.Clin Diagn Lab Immunol. 1995 Sep;2(5):549-53. doi: 10.1128/cdli.2.5.549-553.1995. Clin Diagn Lab Immunol. 1995. PMID: 8548533 Free PMC article.
-
Inhibitory effects of pentoxifylline on LPS-induced leukocyte adhesion and macromolecular extravasation in the microcirculation.Inflamm Res. 1995 Jul;44(7):281-6. doi: 10.1007/BF02032569. Inflamm Res. 1995. PMID: 8564524
-
Effects of pentoxifylline on equine neutrophil function and flow properties.Can J Vet Res. 1992 Oct;56(4):313-7. Can J Vet Res. 1992. PMID: 1335832 Free PMC article.
-
Pentoxifylline suppresses interleukin-2-mediated activation of immature human natural killer cells by inhibiting endogenous tumor necrosis factor-alpha secretion.J Clin Immunol. 1994 Jan;14(1):31-8. doi: 10.1007/BF01541173. J Clin Immunol. 1994. PMID: 8132735
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources