A cleavage map of the TOL plasmid of Pseudomonas putida mt-2
- PMID: 231727
- DOI: 10.1007/BF00267270
A cleavage map of the TOL plasmid of Pseudomonas putida mt-2
Abstract
A cleavage map of the TOL plasmid pWWO has been determined for the restriction endonucleases HindIII and XhoI. A number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified XhoI fragments to Southern blots of HindIII digest products and (iii) analysis of a number of deletion mutants.
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