doi: 10.1093/nar/7.7.1931.
New genes and promoters suggested by the DNA sequence near the end of the coliphage T7 early operon
- PMID: 231766
- PMCID: PMC342357
- DOI: 10.1093/nar/7.7.1931
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New genes and promoters suggested by the DNA sequence near the end of the coliphage T7 early operon
Nucleic Acids Res.
.
Free PMC article
Abstract
We have employed the dideoxynucleotide chain-terminating method to determine the nucleotide sequence of T7 DNA between the physical map positions 18.9% and 19.8%. The most striking features of this sequence are two perfect 21-basepair repeats, each of which appears to contain a promoter for late transcription. In each case the promoter sequence incorporates a putative translational terminator on its left (5'-side of the "sense" strand), and overlaps a potential ribosome-binding site on its right. The region probably lies immediately distal to the early operon, and may contain two short, hitherto unreported protein-coding sequences.
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