Nano - drug Delivery of Apoptosis Activator 2 to AGS Cells by Liposomes Conjugated with Anti-TROP2 Antibody

Abstract

Background: Gastric cancer is the second most common causes of cancer related death in the world and is responsible for two third of cancer related death in the developing countries. survival rate surgery is low and radiation therapy and chemotherapy as alternatives ways for treatment of gastric cancer are not very promising. Thus there is an urgent need for introducing novel treatment procedures and promising new anti-canceric drugs.

Aim: In this study we used pre-prepared liposomes and after necessary manipulations ,these modified liposomes were used for delivery of apoptosis activator 2 to gastric adenocarcinoma cell line (AGS).

Materials and methods: we used pre-prepared liposomes and after necessary manipulations, these modified liposomes were used for delivery of apoptosis activator 2 to AGS cells and induced apoptosis was evaluated by related apoptotic DNA ladder, TUNNEL and Cell Death experiments.

Results: Evaluation of apoptosis by Apoptotic DNA Ladder in liposome treated and untreated AGS cells by DNA laddering and fragmentation, TUNEL and Cell Death Detection confirmed that treatment of AGS cell lines with apoptosis activator 2 loaded liposomes which targeted cell surface TROP2 antigen in cancer cells significantly increased apoptosis in these cells.

Conclusion: Nano drug delivery of apoptosis activator 2 to human gastric adenocarcinoma cell line with liposomes targeted TROP2 antigen is a possible way for smart killing of human gastric adenocarcinoma cells.

Keywords: Apoptosis; Cancer; Human Stomach Adenocarcinoma; Liposome; Tumor-associated calcium signal transducer 2.

Figure 1

Line 1: Untreated AGS cells. Line 2 and 3: 4 and 2.5 mM selenite sodium. Line 4: Control positive of the kit. Line 6 and 7: TROP2 conjugated liposomes loaded with apoptosis activator of double-check experiments

Figure 2

(1) 1.5 mM selenite sodium/ml of cell culture media. (2) 2.5 mM selenite sodium/ml of cell culture media. (3) 4 mM selenite sodium/ml of cell culture media. (4) Empty liposome. (5) untreated AGS. (6) Anti TROP2 conjugated liposomes loaded with apoptosis activator 2. Extent of apoptosis was measured by a nucleosome ELISA Cell Death Detection Kit as described in Materials and Methods section

Figure 3

Induction of apoptosis by anti TROP2 conjugated liposomes loaded with apoptosis activator 2 on AGS cell line. A representative TUNEL staining of AGS cells transfected with anti TROP2-conjugated liposomes loaded with apoptosis activator 2 (right) and control positive of the test (left) were shown