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. 2012 Nov 19:(69):e50053.
doi: 10.3791/50053.

Thinned-skull cortical window technique for in vivo optical coherence tomography imaging

Jenny I Szu  1 , Melissa M Eberle, Carissa L Reynolds, Mike S Hsu, Yan Wang, Christian M Oh, M Shahidul Islam, B Hyle Park, Devin K Binder
Affiliations

Thinned-skull cortical window technique for in vivo optical coherence tomography imaging

Jenny I Szu et al. J Vis Exp. .

Abstract

Optical coherence tomography (OCT) is a biomedical imaging technique with high spatial-temporal resolution. With its minimally invasive approach OCT has been used extensively in ophthalmology, dermatology, and gastroenterology. Using a thinned-skull cortical window (TSCW), we employ spectral-domain OCT (SD-OCT) modality as a tool to image the cortex in vivo. Commonly, an opened-skull has been used for neuro-imaging as it provides more versatility, however, a TSCW approach is less invasive and is an effective mean for long term imaging in neuropathology studies. Here, we present a method of creating a TSCW in a mouse model for in vivo OCT imaging of the cerebral cortex.

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