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. 2013 Jan;2(1):114-8.
doi: 10.1002/adhm.201200115. Epub 2012 Sep 28.

Sequence-specific crosslinking of electrospun, elastin-like protein preserves bioactivity and native-like mechanics

Patrick L Benitez  1 , Jeffrey A Sweet, Helen Fink, Krishna P Chennazhi, Shantikumar V Nair, Annika Enejder, Sarah C Heilshorn
Affiliations

Sequence-specific crosslinking of electrospun, elastin-like protein preserves bioactivity and native-like mechanics

Patrick L Benitez et al. Adv Healthc Mater. 2013 Jan.
No abstract available

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Figures

Figure 1
Figure 1
Stability of nanofibrous ELP. a) Comparison of protein loss in physiological conditions over eight days from untreated fabrics and two-stage crosslinked fabrics. b) CARS microscopy (2930 cm−1) 3D view of nanofibers after two weeks in physiological buffer. c) Schematic of two-stage crosslinking strategy. Crosslinking is initiated by exposing matrices to glutaraldehyde vapor. Complete crosslinks are formed after hydration in a concentrated sodium chloride buffer. d) LCST of ELP in varying buffer conditions. e) Comparison of overnight protein loss at physiological conditions for fabrics hydrated in varying w/v, ELP per 10× phosphate-buffered saline (PBS), during the aqueous crosslinking completion step.
Figure 2
Figure 2
Physical characterization of electrospun ELP. a) SEM of fibers spun from aqueous solutions of a) 20%, and b) 35% w/w ELP. c) Histograms and Gaussian fits of nanofiber width and thickness (n > 100) from both solutions. d) An implantable bulk fabric in PBS, and e) a representative tensile stress-strain curve.
Figure 3
Figure 3
Bioactivity of the RGD ligand. a) Metabolic activity of rMSCs after 24 h in serum-free medium on ELP-RGD (8.4 mM RGD) or ELP-RDG (0 mM RGD) fabrics or glass control (n = 3). Maximal projection images obtained by CARS microscopy of rMSC after 24 h in standard conditions on electrospun ELP-RGD: b) nanofibers, c) rMSCs, d) overlay. Close contact is suggested by imprints of nanofibers on the cell membrane, white arrow.
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