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. 2012 Nov;1(6):785-9.
doi: 10.1002/adhm.201200195. Epub 2012 Sep 5.

Protein-engineered biomaterials to generate human skeletal muscle mimics

Debanti Sengupta  1 , Penney M Gilbert, Kyle J Johnson, Helen M Blau, Sarah C Heilshorn
Affiliations

Protein-engineered biomaterials to generate human skeletal muscle mimics

Debanti Sengupta et al. Adv Healthc Mater. 2012 Nov.
No abstract available

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Figures

Figure 1
Figure 1
A. Representative micrographs of primary human myoblasts cultured on protein-engineered biomaterials. RGD density varies from 930 to 9300 ligands/μm2 and groove spacing varies from 20 to 200 μm. Micropattern topography is visualized by phase contrast microscopy (top) and as dashed white lines on fluorescent micrographs (bottom). Myoblasts are stained for myogenin (muscle cell marker, red) and counter-stained with DAPI (nuclear marker, blue). B. Representative quantification of cell morphology. Area within the perimeter is the total spread area (left). Each perimeter is computationally approximated by a best-fit ellipse with major (X) and minor (Y) axes to quantify the aspect ratio (X/Y, center). The angle between the cell major axis (X) and the micropattern determines cell alignment (right).
Figure 2
Figure 2
Human myoblast (A,C) and C2C12 cell (B,D) alignment, spread area, and aspect ratio in response to biomaterial microtopographical groove spacing (A,B) and cell-adhesive RGD ligand density (C,D). Data shown as box-and-whisker plots: box marks the 25th to 75th percentiles, horizontal line marks the median, whiskers mark the 5th to 95th percentiles. Letters over the bars represent statistical significance with a 99.9% confidence interval; bars with the same letters are statistically similar, while bars with different letters are statistically different.
Figure 3
Figure 3
Human myotube differentiation on protein-engineered biomaterials. A. Fluorescent micrographs of myotubes stained for smooth muscle myosin heavy chain (SMMHC, green, left) or a-actinin, a marker of sarcomeric assembly (green, right) and counter-stained with DAPI nuclear marker (blue). B. Human myotube alignment, nuclear index, length, and width were measured as a function of microtopographical groove spacing. Box-and-whisker plots shown as in Figure 2; the nuclear index plot shows each individual myotube as a separate data point with the population median indicated by a red line.
See this image and copyright information in PMC

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