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. 2013 Jan;15(1):28-39.
doi: 10.1038/ncb2632. Epub 2012 Dec 2.

Anthrax toxin receptor 2a controls mitotic spindle positioning

I Castanon  1 , L Abrami, L Holtzer, C P Heisenberg, F G van der Goot, M González-Gaitán
Affiliations

Anthrax toxin receptor 2a controls mitotic spindle positioning

I Castanon et al. Nat Cell Biol. 2013 Jan.

Abstract

Oriented mitosis is essential during tissue morphogenesis. The Wnt/planar cell polarity (Wnt/PCP) pathway orients mitosis in a number of developmental systems, including dorsal epiblast cell divisions along the animal-vegetal (A-V) axis during zebrafish gastrulation. How Wnt signalling orients the mitotic plane is, however, unknown. Here we show that, in dorsal epiblast cells, anthrax toxin receptor 2a (Antxr2a) accumulates in a polarized cortical cap, which is aligned with the embryonic A-V axis and forecasts the division plane. Filamentous actin (F-actin) also forms an A-V polarized cap, which depends on Wnt/PCP and its effectors RhoA and Rock2. Antxr2a is recruited to the cap by interacting with actin. Antxr2a also interacts with RhoA and together they activate the diaphanous-related formin zDia2. Mechanistically, Antxr2a functions as a Wnt-dependent polarized determinant, which, through the action of RhoA and zDia2, exerts torque on the spindle to align it with the A-V axis.

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References

    1. J Neurosci. 1994 Jun;14(6):3475-86 - PubMed
    1. Biophys J. 2007 Jan 15;92(2):654-60 - PubMed
    1. Angiogenesis. 2008;11(4):347-60 - PubMed
    1. Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):751-6 - PubMed
    1. Curr Biol. 2008 Oct 14;18(19):1514-9 - PubMed

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