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. 2012 Nov 5;101(19):193701.
doi: 10.1063/1.4766191. Epub 2012 Nov 6.

A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture

Zhengbao Zha  1 , Linan Jiang, Zhifei Dai, Xiaoyi Wu
Affiliations

A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture

Zhengbao Zha et al. Appl Phys Lett. .

Abstract

The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.

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Figures

Figure 1
Figure 1
A proposed model for fiber formation and antibody immobilization. (a) CSS chemical structure. (b) A schematic representation of spherical micelle, cylindrical micelle, and nanofiber formation, and an insert-and-tighten mechanism for the stable and functional immobilization of membrane-bound molecules such as antibodies.
Figure 2
Figure 2
Hydration-induced diameter reduction of CSS nanofibers. (a) SEM images of as-spun CSS nanofibers and CSS nanofibers that were hydrated in 1x PBS for 72 h. (b) Diameter distribution of as-spun and hydrated CSS nanofibers.
Figure 3
Figure 3
Functional immobilization of membrane-bound molecules on CSS nanofibers. (a) NBD-DPPE. (b) and (c) Anti-CD 20 Abs. Immunofluorecent (b) and SEM images (c) of Granta-22 cells captured on the anti-CD20 functionalized CSS nanofiber meshes. The cells were fixed and stained for nucleus with DAPI in blue and for actin with phalloidin in red.
Figure 4
Figure 4
A control study using CSS films. (a) Optical image of CSS films. Cracks (in black) appeared on the films. Immunofluorecent image of Granta-22 cells captured on the non-functionalized (b) and anti-CD20 functionalized CSS films (c). The cells were fixed and stained for nucleus with DAPI.
See this image and copyright information in PMC

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