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. 1979 Oct;32(1):72-7.
doi: 10.1128/JVI.32.1.72-77.1979.

Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate

P A Furman, M H St Clair, J A Fyfe, J L Rideout, P M Keller, G B Elion

Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate

P A Furman et al. J Virol. 1979 Oct.

Abstract

The effect of the nucleoside analog 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine) on herpes simplex virus type 1 DNA synthesis was examined. Acycloguanosine inhibited herpesvirus DNA synthesis in virus-infected cells. The synthesis of host cell DNA was only partially inhibited in actively growing cells at acycloguanosine concentrations several hundred-fold greater than the 50% effective dose for herpes simplex virus type 1. Studies using partially purified enzymes revealed that the triphosphate of this compound inhibited the virus-induced DNA polymerases (DNA nucleotidyltransferases) to a greater degree than the DNA polymerase of the host cell, that the inhibition was dependent upon the base composition of the template, and that the triphosphate was a better substrate for the virus-induced polymerases than for the alpha cellular DNA polymerases.

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References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5716-20 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4684-8 - PubMed
    1. J Biol Chem. 1978 Dec 25;253(24):8721-7 - PubMed
    1. Nature. 1978 Apr 13;272(5654):583-5 - PubMed
    1. Antimicrob Agents Chemother. 1976 Jul;10(1):119-22 - PubMed

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