doi: 10.1016/j.mimet.2012.11.020.
Epub 2012 Dec 5.
Rapid and sensitive detection of Acinetobacter baumannii using loop-mediated isothermal amplification
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- PMID: 23220188
- DOI: 10.1016/j.mimet.2012.11.020
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Rapid and sensitive detection of Acinetobacter baumannii using loop-mediated isothermal amplification
J Microbiol Methods.
.
Abstract
Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Acinetobacter baumannii DNA based on the 16S-23S rRNA intergenic spacer (ITS) sequence. The results showed that target DNA was amplified and visualized within 30min and with a detection limit 100-fold greater than PCR.
Copyright © 2013 Elsevier B.V. All rights reserved.
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