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. 2013 Feb 15;92(2):197-200.
doi: 10.1016/j.mimet.2012.11.020. Epub 2012 Dec 5.

Rapid and sensitive detection of Acinetobacter baumannii using loop-mediated isothermal amplification

Po-Chi Soo  1 , Chun-Chieh Tseng, Siao-Ru Ling, Ming-Li Liou, Chih-Chin Liu, Huei-Jen Chao, Teng-Yi Lin, Kai-Chih Chang
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Rapid and sensitive detection of Acinetobacter baumannii using loop-mediated isothermal amplification

Po-Chi Soo et al. J Microbiol Methods. .

Abstract

Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Acinetobacter baumannii DNA based on the 16S-23S rRNA intergenic spacer (ITS) sequence. The results showed that target DNA was amplified and visualized within 30min and with a detection limit 100-fold greater than PCR.

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