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. 2012 Nov 13;24(21):4008-4010.
doi: 10.1021/cm302582z. Epub 2012 Oct 19.

Coating Evaluation and Purification of Monodisperse, Water-Soluble, Magnetic Nanoparticles Using Sucrose Density Gradient Ultracentrifugation

Andrew M Prantner  1 , Jun Chen, Christopher B Murray, Nathalie Scholler
Affiliations

Coating Evaluation and Purification of Monodisperse, Water-Soluble, Magnetic Nanoparticles Using Sucrose Density Gradient Ultracentrifugation

Andrew M Prantner et al. Chem Mater. .
No abstract available

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Figures

Figure 1
Figure 1. Water-soluble nanoparticles prepared by dry-film hydration
(A) Transmission electron microscopy image of oleic acid stabilized cobalt ferrite nanocrystals. Digital camera images of (B) nanoparticles prepared by the dry-film protocol and (C) empty phospholipid micelles labeled with PKH26 after separation by sucrose density gradient ultracentrifugation. The markings in (B and C) indicate the approximate position of the density gradient steps (water, 5, 30, 40, 50, 60, 70, and 80% sucrose).
Figure 2
Figure 2. Water-soluble nanoparticles prepared by solvent exchange
Digital camera images of sucrose density gradient ultracentrifugation separation of nanoparticle samples containing either (A) 175 μg or (B and C) of 500 μg of total iron. (C) is a top view of (B). The markings in (B) indicate the approximate position of the density gradient steps (water, 30, 40, 50, 60, 70, and 80% sucrose).
Figure 3
Figure 3. Qualitative evaluation of nanoparticle preparations
Samples were initially prepared at an iron to DSPE-PEG-2000 weight ratio of i) 1:5, ii) 1:10, and iii) 1:20 by solvent exchange. (A) Samples were analyzed by agarose gel electrophoresis (0.6%, 100 V, 60 min) prior to (B) sucrose gradient ultracentrifugation. The markings in (B) indicate the approximate position of the density gradient steps (water, 30, 40, 50, 60, 70, and 80% sucrose).
See this image and copyright information in PMC

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