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. 2013 Feb;51(2):647-52.
doi: 10.1128/JCM.02927-12. Epub 2012 Dec 5.

Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden

Fabiana C Pimenta  1 , Alexis Roundtree, Ahmet Soysal, Mustafa Bakir, Mignon du Plessis, Nicole Wolter, Anne von Gottberg, Lesley McGee, Maria da Gloria Carvalho, Bernard Beall
Affiliations

Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden

Fabiana C Pimenta et al. J Clin Microbiol. 2013 Feb.

Abstract

We developed and validated a real-time PCR assay consisting of 7 triplexed reactions to identify 11 individual serotypes plus 10 small serogroups representing the majority of disease-causing isolates of Streptococcus pneumoniae. This assay targets the 13 serotypes included within the 13-valent conjugate vaccine and 8 additional key serotypes or serogroups. Advantages over other serotyping assays are described. The assay will be expanded to 40 serotypes/serogroups. We will provide periodic updates at our protocol website.

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References

    1. Carvalho MDG, Pimenta FC, Jackson D, Roundtree A, Ahmad Y, Millar EV, O'Brien KL, Whitney CG, Cohen AL, Beall BW. 2010. Revisiting pneumococcal carriage using broth-enrichment and PCR techniques for enhanced detection of carriage and serotypes. J. Clin. Microbiol. 48:1611–1618 - PMC - PubMed
    1. Jin P, Xiao M, Kong F, Oftadeh S, Zhou F, Liu C, Gilbert GL. 2009. Simple, accurate, serotype-specific PCR assay to differentiate Streptococcus pneumoniae serotypes 6A, 6B, and 6C. J. Clin. Microbiol. 47:2470–2474 - PMC - PubMed
    1. Kong F, Brown M, Sabananthan A, Zeng X, Gilbert GL. 2006. Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes. J. Clin. Microbiol. 44:1887–1891 - PMC - PubMed
    1. Zhou F, Kong F, Tong Z, Gilbert GL. 2007. Identification of less-common Streptococcus pneumoniae serotypes by a multiplex PCR-based reverse line blot hybridization assay. J. Clin. Microbiol. 45:3411–3415 - PMC - PubMed
    1. Ansaldi F, de Florentiis D, Canepa P, Zancolli M, Martini M, Orsi A, Durando P, Icardi G. 2012. Carriage of Streptococcus pneumoniae 7 years after implementation of vaccination program in a population with very high and long-lasting coverage, Italy. Vaccine 30:2288–2294 - PubMed

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