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. 2013 Apr 15:42:434-8.
doi: 10.1016/j.bios.2012.10.080. Epub 2012 Nov 16.

A carbon nanofiber based biosensor for simultaneous detection of dopamine and serotonin in the presence of ascorbic acid

Emily Rand  1 , Adaikkappan Periyakaruppan, Zuki Tanaka, David A Zhang, Michael P Marsh, Russell J Andrews, Kendall H Lee, Bin Chen, M Meyyappan, Jessica E Koehne
Affiliations

A carbon nanofiber based biosensor for simultaneous detection of dopamine and serotonin in the presence of ascorbic acid

Emily Rand et al. Biosens Bioelectron. .

Abstract

A biosensor based on an array of vertically aligned carbon nanofibers (CNFs) grown by plasma enhanced chemical vapor deposition is found to be effective for the simultaneous detection of dopamine (DA) and serotonin (5-HT) in the presence of excess ascorbic acid (AA). The CNF electrode outperforms the conventional glassy carbon electrode (GCE) for both selectivity and sensitivity. Using differential pulse voltammetry (DPV), three distinct peaks are seen for the CNF electrode at 0.13 V, 0.45 V, and 0.70 V for the ternary mixture of AA, DA, and 5-HT. In contrast, the analytes are indistinguishable in a mixture using a GCE. For the CNF electrode, the detection limits are 50 nM for DA and 250 nM for 5-HT.

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Figures

Fig. 1
Fig. 1
a) SEM image of an array of vertically aligned CNFs. Scale bar is 1μm. b) TEM image of an individual nanofiber. Arrows highlight active sites. Scale bar is 0.1 μm. c) Raman spectroscopy of carbon nanofibers. d) FT-IR spectroscopy of carbon nanofibers.
Fig. 2
Fig. 2
a) Baseline-corrected DPV scans of CNF electrode with DA concentrations 10 μM, 5 μM, 1 μM, 0.5 μM, 0.1 μM, 0.05 μM; b) Calibration curve for DA; c) Baseline-corrected DPV scans of CNF electrode with 5-HT concentrations 10 μM, 5 μM, 1 μM, 0.5 μM, 0.25 μM, 0.1 μM; and d) Calibration curve for 5-HT
Fig. 3
Fig. 3
a) Baseline-corrected DPV plots of individual detection of 10 μM DA, 1 mM AA, and 10 μM 5-HT with a glassy carbon electrode; b) Background subtracted DPV plots of individual detection of 10 μM DA, 1 mM AA, and 10 μM 5-HT with a carbon nanofiber electrode; c) Baseline-corrected DPV plots of a ternary mixture of 10 uμM DA, 1 mM AA, and 10 μM 5-HT with a glassy carbon electrode; d) Baseline-corrected DPV plots of a ternary mixture of 1 mM AA, 10 μM DA, and 5-HT (10 μM, 5 μM, 2.5 μM, 1 μM, 0.5 μM, 0.25 μM) with a carbon nanofiber electrode; e) Baseline-corrected DPV plots of a ternary mixture of 1 mM AA, 10 μM 5-HT, and DA (10 μM, 5 μM, 2.5 μM, 1 μM, 0.5 μM, 0.25 μM, 0.1 μM) with a carbon nanofiber electrode
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