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. 2012 Dec 11;43(1):84.
doi: 10.1186/1297-9716-43-84.

Schmallenberg virus challenge models in cattle: infectious serum or culture-grown virus?

Kerstin Wernike  1 , Michael Eschbaumer, Angele Breithaupt, Bernd Hoffmann, Martin Beer
Affiliations

Schmallenberg virus challenge models in cattle: infectious serum or culture-grown virus?

Kerstin Wernike et al. Vet Res. .

Abstract

Schmallenberg virus (SBV), discovered in Europe in 2011, causes mild transient disease in adult ruminants, but fetal infection can lead to severe malformation in cattle, sheep and goats.To elucidate the pathogenesis of this novel orthobunyavirus, considerable efforts are required. A reliable and standardized infection model is essential for in vivo studies. In the present study, two groups of four cattle were inoculated with either serum passaged in cattle only or cell culture-grown virus. The replication of culture-grown SBV in cattle was reduced compared to virus inoculated via infectious serum. In a second experiment, the infectious serum was titrated in calves; the tested batch contained 102.83 infectious doses per mL. Hence, serum-borne virus that was only passaged in the natural host is a suitable option for a standardized SBV infection model.

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Figures

Figure 1
Figure 1
Real-time RT-PCR results of cattle inoculated with infectious serum or cell culture supernatant. All cattle, either inoculated with infectious serum or culture-grown virus, scored positive in the RT-qPCR for several days. In the in-contact animals SBV RNA was not detected at any time.
Figure 2
Figure 2
Real-time RT-PCR results of cattle in the in-vivo titration experiment. All animals inoculated with the undiluted serum, dilution 1/10 and 1/100 were positive for several days. Only one (C25) out of three animals inoculated with dilution 1/1000 scored positive.
See this image and copyright information in PMC

References

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