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. 2012 Dec 6;17(49):20334.
doi: 10.2807/ese.17.49.20334-en.

Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections

V M Corman  1 , M A Müller, U Costabel, J Timm, T Binger, B Meyer, P Kreher, E Lattwein, M Eschbach-Bludau, A Nitsche, T Bleicker, O Landt, B Schweiger, J F Drexler, A D Osterhaus, B L Haagmans, U Dittmer, F Bonin, T Wolff, C Drosten
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Free article

Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections

V M Corman et al. Euro Surveill. .
Free article

Abstract

We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.

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