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. 2012 Jun 30;25(2):66-73.

The efficacy of recombinant human activated protein C (rhAPC) vs antithrombin III (at III) vs heparin, in the healing process of partial-thickness burns: a comparative study

O Kritikos  1 , M Tsagarakis, D Tsoutsos, C Kittas, V Gorgoulis, A Papalois, A Giannopoulos, G Kakiopoulos, O Papadopoulos
Affiliations

The efficacy of recombinant human activated protein C (rhAPC) vs antithrombin III (at III) vs heparin, in the healing process of partial-thickness burns: a comparative study

O Kritikos et al. Ann Burns Fire Disasters. .

Abstract
in English, French

This is an experimental study regarding the positive effect of recombinant human activated protein C (rhAPC) in the healing process of partial-thickness burns, in comparison to antithrombin III and heparin. On a porcine model we induced superficial partial-thickness and deep partial-thickness burns and performed intravenous administration of the elements of study during the first 48 h. The progress of the condition of the injured tissues was evaluated by histopathological examination at specific time intervals. The results showed an improved healing response of the specimens treated with rhAPC compared to those treated with antithrombin III, heparin, and placebo.

Les Auteurs proposent une étude expérimentale sur l’effet positif de la protéine recombinante humaine activée C (PrhAC) dans le processus de la guérison des brûlures d’épaisseur partielle, par rapport à l’antithrombine III et à l’héparine. Sur un modèle porcin ils ont induit des brûlures superficielles d’épaisseur partielle et d’épaisseur profondes. En outre ils ont effectué l’administration intraveineuse des éléments d’étude au cours des 48 premières heures. L’évolution de l’état des tissus lésés a été évaluée par un examen histopathologique à intervalles de temps spécifiques. Les résultats ont montré que les échantillons traités avec PrhAC manifestaient une guérison supérieure par rapport aux échantillons traités avec l’antithrombine III, l’héparine et le placebo.

Keywords: burns; healing; recombinant human activated protein C (rhAPC).

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Figures

Fig. 1
Fig. 1. Creation of burn wound.
Table I
Table I. The 24 pigs were divided into four groups. Each of the elements of study was treated intravenously starting after completion of burn injuries
Fig. 2
Fig. 2. Intubated pigs lying on dorsum. Markings made for catheterization procedure.
Fig. 3
Fig. 3. Cannulation of jugular vein.
Fig. 4
Fig. 4. Continuous infusion for 48 h
Fig. 5
Fig. 5. Normal skin. The skin of the back of the pig at this age measured approximately 1.35 mm with a keratine layer of approximately 0.15 mm and a cutis of approximately 1.17 mm.
Fig. 6
Fig. 6. Superficial partialthickness burn (average depth, 0.73 mm).
Fig. 7
Fig. 7. Deep partial-thickness burn (average depth, 0.95 mm).
Fig. 8
Fig. 8. rhAPC group, day 13 post-burn.
Fig. 9
Fig. 9. Heparin group, day 13 post-burn.
Fig. 10
Fig. 10. Placebo group, day 13 post-burn.
Fig. 11
Fig. 11. AT III group, day 13 post-burn.
Table II
Table II. Healing effect in superficial partial-thickness burns indicated by histological evaluation of wound depth (WD)
Table III
Table III. Healing effect in deep partial-thickness burns indicated by histological evaluation in wound depth (WD)
Chart 1
Chart 1. Healing effect of the substances studied in superficial partial-thickness burns by assessment of depth of total burn injury in time.
Chart 2
Chart 2. Healing effect of the study substances in deep partialthickness burns by assessment of depth in total burn injury in time.
See this image and copyright information in PMC

References

    1. Regas FC, Erlich HP. Elucidating the vascular response to burns with a new rat model. J Trauma. 1992;32:557. - PubMed
    1. Cetinkale O, Demir M, Sayman HB, et al. Effects of allopurinol, ibuprofen and cyclosporine A on local microcirculatory disturbance due to burn injuries. Burns. 1997;23:43. - PubMed
    1. Bucky LP, Vedder NB, Hong HZ, et al. Reduction of burn injury by inhibiting CD 18-mediated leukocyte adherence in rabbits. Plast Reconstr Surg. 1994;93:1473. - PubMed
    1. Hashimoto I, Nakanishi H, Shono Y, et al. The features of thrombus in a microvessel injury model and the antithrombotic efficacy of heparin, urokinase and prostaglandin E1. Plast Reconstr Surg. 2003;111:2307–14. - PubMed
    1. Danielson P, Nilsson L, Nettelbland H. Is there a need for antithrombin III substitution early after burn injury? Burns. 1997;23:300–5. - PubMed

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