Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Dec;158(4):552-6.
doi: 10.5507/bp.2012.106. Epub 2012 Dec 10.

Retinal toxicity after repeated intravitreal carboplatin injection into rabbit eyes

Pavel Pochop  1 , Denisa Darsova, Jiri Uhlik, Jan Lestak, Jiri Kukacka, Daniela Kodetova, Eva Klapkova, Josef Malis, Ludek Vajner
Affiliations
Free article

Retinal toxicity after repeated intravitreal carboplatin injection into rabbit eyes

Pavel Pochop et al. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014 Dec.
Free article

Abstract

Background: The aim of this study was to assess retinal toxicity in a rabbit model after carboplatin delivered as repeated transcorneal intravitreal injection, in order to determine the highest possible safe dose for use in human retinoblastoma "seeding" tumor chemotherapy.

Methods and results: We used six albino rabbits in an in vivo experiment and injected 0.008 mg of carboplatin intravitreally (iv) 4 times at two-week intervals. 0.08 mL saline was injected into the left eye. We recorded electroretinograms (ERGs) before the first carboplatin injection and after the fourth injection. Platinum concentration was measured 1 h after the fifth additional injection. We found reduced dark-adapted b-wave amplitudes and, light-adapted b-wave and a-wave amplitudes. The differences between right and left eyes was significant but we found no histopathologic retinal changes.

Conclusions: 0.008 mg of carboplatin is probably the highest possible safe dose for the treatment of retinoblastoma patients. Questionable is direct extrapolation of retinal toxicity from the rabbit eye model to the human eye.

PubMed Disclaimer