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. 2012 Dec 18:10:46.
doi: 10.1186/1477-3155-10-46.

Synthesis, characterization and in vitro studies of doxorubicin-loaded magnetic nanoparticles grafted to smart copolymers on A549 lung cancer cell line

Abolfazl Akbarzadeh  1 , Mohammad Samiei, Sang Woo Joo, Maryam Anzaby, Younes Hanifehpour, Hamid Tayefi Nasrabadi, Soodabeh Davaran
Affiliations

Synthesis, characterization and in vitro studies of doxorubicin-loaded magnetic nanoparticles grafted to smart copolymers on A549 lung cancer cell line

Abolfazl Akbarzadeh et al. J Nanobiotechnology. .

Retraction in

Abstract

Background: The aim of present study was to develop the novel methods for chemical and physical modification of superparamagnetic iron oxide nanoparticles (SPIONs) with polymers via covalent bonding entrapment. These modified SPIONs were used for encapsulation of anticancer drug doxorubicin.

Method: At first approach silane-grafted magnetic nanoparticles was prepared and used as a template for polymerization of the N-isopropylacrylamide (NIPAAm) and methacrylic acid (MAA) via radical polymerization. This temperature/pH-sensitive copolymer was used for preparation of DOX-loaded magnetic nanocomposites. At second approach Vinyltriethoxysilane-grafted magnetic nanoparticles were used as a template to polymerize PNIPAAm-MAA in 1, 4 dioxan and methylene-bis-acrylamide (BIS) was used as a cross-linking agent. Chemical composition and magnetic properties of Dox-loaded magnetic hydrogel nanocomposites were analyzed by FT-IR, XRD, and VSM.

Results: The results demonstrate the feasibility of drug encapsulation of the magnetic nanoparticles with NIPAAm-MAA copolymer via covalent bonding. The key factors for the successful prepardtion of magnetic nanocomposites were the structure of copolymer (linear or cross-linked), concentration of copolymer and concentration of drug. The influence of pH and temperature on the release profile of doxorubicin was examined. The in vitro cytotoxicity test (MTT assay) of both magnetic DOx-loaded nanoparticles was examined. The in vitro tests showed that these systems are no toxicity and are biocompatible.

Conclusion: IC50 of DOx-loaded Fe3O4 nanoparticles on A549 lung cancer cell line showed that systems could be useful in treatment of lung cancer.

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Figures

Figure 1
Figure 1
Applications of superparamagnetic iron oxide nanoparticles (SPION)10.
Figure 2
Figure 2
Structure of the PNIPAAm-MAA copolymer.
Figure 3
Figure 3
Magnetite-hexane suspension attached to a magnet.
Figure 4
Figure 4
(A) Chemical modification of Fe3O4 surface by grafting polymerization without cross-linking (B) In presence of cross-linking.
Figure 5
Figure 5
XRD patterns of (a) pure Fe3O4 nanoparticles.
Figure 6
Figure 6
The SEM micrographs of (a) pure Fe3O4 nanoparticles (b) Fe3O4 nanoparticles grafted by poly-(NIPAAm-MMA) (c) Hydrodynamic sizes of PNIPAAm-MAA-grafted MNPs.
Figure 7
Figure 7
FT-IR spectra of (a) pure Fe3O4 nanoparticles, (b) Fe3O4 nanoparticles modified by VTES, (c) poly(NIPAAm-MMA)-grafted Fe3O4 nanoparticles.
Figure 8
Figure 8
The magnetic behavior of magnetic nanoparticles. (1.Fe3O4, 2. VTES-Fe3O4, 3. VTES-Fe3O4-PNIPAAm-MAA).
Figure 9
Figure 9
Release profiles of doxorubicin from the hybrid nanoparticles at different pH /temperature values.
Figure 10
Figure 10
IC50 of (a) the doxorubicin-loaded linear PNIPAAm-MAA-grafted magnetic nanoparticles (b) the doxorubicin-loaded cross linker PNIPAAm-MAA-grafted magnetic nanoparticles (c) Pure doxorubicin on A549 tumor cell line after 24, 48 and 72 h treatment.
See this image and copyright information in PMC

References

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