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. 2012 Nov-Dec;44(6):683-7.
doi: 10.4103/0253-7613.103242.

Protective effect of methanolic extract of Garcinia indica fruits in 6-OHDA rat model of Parkinson's disease

Bhaveshkumar V Antala  1 , Manishkumar S Patel, Satish V Bhuva, Shiv Gupta, Samir Rabadiya, Mangala Lahkar
Affiliations

Protective effect of methanolic extract of Garcinia indica fruits in 6-OHDA rat model of Parkinson's disease

Bhaveshkumar V Antala et al. Indian J Pharmacol. 2012 Nov-Dec.

Abstract

Context: Several studies have reported that antioxidants play an important role in Parkinson's disease (PD). Garcinia indica extract is a natural antioxidant, the present study was undertaken to evaluate the neuroprotective effect of methanolic extract of Garcinia indica (GIM) against 6-hydroxydopamine (6-OHDA) neurotoxicity for striatal dopaminergic neurons in the rat.

Materials and methods: Thirty adult Wistar rats were randomly divided into five groups namely control, 6-OHDA model, and GIM (100, 200, and 400 mg/kg body weight suspended in one ml of 0.1% carboxymethyl cellulose). The treatment was started three days before surgery and continued for next 14 days. The surgery was done on third day in all groups for administration of 6-OHDA into the right striatum and right substantia nigra, whereas control group injected with 6-OHDA vehicle. Various behavior and biochemical tests (Apomorphine-induced rotational behavior, Stepping test, Initiation time, Postural balance test, and Disengage time) were used to evaluate the neuroprotective effect of GIM. One-way analysis of variance (ANOVA) followed by Dunnett's test was used to compare inter-group differences. P<0.05 was considered as statistically significant.

Results: GIM had significant (P<0.05, P<0.01) preventive effect in biochemical tests, i.e., dopamine and its metabolites measurement and in various behavior tests, i.e., apomorphine-induced rotational behavior, stepping test, initiation time, postural balance test, and disengage time as compared to 6-OHDA-treated rats.

Conclusions: Our results demonstrated that GIM acted as an effective neuroprotective agent for striatal dopaminergic neurons in 6-OHDA lesioned rat model of PD.

Keywords: 6-OHDA; Garcinia indica; Parkinson's disease.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Effect of GIM on amphetamine-induced rotation test in 6-OHDA rat model for Parkinson's Disease Values are expressed as mean ± SEM, (n = 6 in each group) *P<0.05, **P<0.01 vs 6-OHDA group, ##P<0.001 vs control group, GIM = methanolic extract of Garcinia indica, 6-OHDA = 6-hydroxydopamine,
See this image and copyright information in PMC

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