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. 2012 Sep;16(3):343-8.
doi: 10.4103/0973-029X.102481.

Immunohistochemical evaluation of mast cells and vascular endothelial proliferation in oral precancerous lesion-leukoplakia

M Sathyakumar  1 , G Sriram, Tr Saraswathi, B Sivapathasundharam
Affiliations

Immunohistochemical evaluation of mast cells and vascular endothelial proliferation in oral precancerous lesion-leukoplakia

M Sathyakumar et al. J Oral Maxillofac Pathol. 2012 Sep.

Abstract

Background: Oral leukoplakia is the best-known precursor lesion. Although a morphologic feature of oral epithelial dysplasia is well described, less is known about the pathobiologic changes within the cells and over the cell surfaces for malignant transformation.

Aims: The present study is aimed at comparing and correlating the mast cell density (MCD) and micro vascular density (MVD) in Normal Mucosa (NM) and different grades of dysplasia and to analyze their role in disease progression.

Materials and methods: MCD was assessed using anti mast cell tryptase and MVD was assessed immunohistochemically using anti-Factor VIII related von Willibrand factor.

Results: The Results of the present study showed an exponential increase in microvessel density as mast cell density increased.

Conclusion: The role of mast cells in angiogenesis as it progresses from normal mucosa to dysplasia is in concordance with the study. The number of mast cells and microvessel can be used as indictors of disease progression.

Keywords: Angiogenesis; mast cells; oral epithelial dysplasia.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Immunohistochemical demonstration of mast cells in normal mucosa using mouse monoclonal anti-mast cell tryptase antibody (×400)
Figure 2
Figure 2
Immunohistochemical demonstration of mast cells in low grade dysplasia using mouse monoclonal anti-mast cell tryptase antibody (×400)
Figure 3
Figure 3
Immunohistochemical demonstration of mast cells in high grade dysplasia using mouse monoclonal anti-mast cell tryptase antibody (×400)
Figure 4
Figure 4
Immunohistochemical demonstration of microvessels in normal mucosa using mouse monoclonal anti-factor VIII related Von willebrand factor antibody (×400)
Figure 5
Figure 5
Immunohistochemical demonstration of microvessels in low grade dysplasia using mouse monoclonal anti-factor VIII related Von willebrand factor antibody (×400)
Figure 6
Figure 6
Immunohistochemical demonstration of microvessels in high grade dysplasia using mouse monoclonal anti-factor VIII related Von willebrand factor antibody (×400)
Figure 7
Figure 7
Scattered diagram showing correlation of MCD and MVD in low grade dysplasia
See this image and copyright information in PMC

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