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Comparative Study
. 1990 Jan 25;18(2):351-3.
doi: 10.1093/nar/18.2.351.

Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure

S Alberti  1 , M Fornaro
Affiliations
Free PMC article
Comparative Study

Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure

S Alberti et al. Nucleic Acids Res. .
Free PMC article

Abstract

Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate. The DNA obtained following the present protocol transfects eukaryotic cells with higher efficiency.

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References

    1. Proc Natl Acad Sci U S A. 1981 Jan;78(1):616-20 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7876-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8391-4 - PubMed
    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
    1. Cell. 1978 Nov;15(3):1003-10 - PubMed

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