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. 2012 Dec 24:5:694.
doi: 10.1186/1756-0500-5-694.

Expression of antigen tf and galectin-3 in fibroadenoma

Itandehui Belem Gallegos  1 , Eduardo Pérez-Campos, Margarito Martinez, Miguel Ángel Mayoral, Laura Pérez, Sergio Aguilar, Edgar Zenteno, Maria del Socorro Pina, Pedro Hernández
Affiliations

Expression of antigen tf and galectin-3 in fibroadenoma

Itandehui Belem Gallegos et al. BMC Res Notes. .

Abstract

Background: Fibroadenomas are benign human breast tumors, characterized by proliferation of epithelial and stromal components of the terminal ductal unit. They may grow, regress or remain unchanged, as the hormonal environment of the patient changes. Expression of antigen TF in mucin or mucin-type glycoproteins and of galectin-3 seems to contribute to proliferation and transformations events; their expression has been reported in ductal breast cancer and in aggressive tumors.

Findings: Lectin histochemistry, immunohistochemistry, and immunofluorescence were used to examine the expression and distribution of antigen TF and galectin-3. We used lectins from Arachis hypogaea, Artocarpus integrifolia, and Amaranthus lecuocarpus to evaluate TF expression and a monoclonal antibody to evaluate galectin-3 expression. We used paraffin-embedded blocks from 10 breast tissues diagnosed with fibroadenoma and as control 10 healthy tissue samples. Histochemical and immunofluorescence analysis showed positive expression of galectin-3 in fibroadenoma tissue, mainly in stroma, weak interaction in ducts was observed; whereas, in healthy tissue samples the staining was also weak in ducts. Lectins from A. leucocarpus and A. integrifolia specificaly recognized ducts in healthy breast samples, whereas the lectin from A. hypogaea recognized ducts and stroma. In fibroadenoma tissue, the lectins from A. integrifolia, A. Hypogaea, and A. leucocarpus recognized mainly ducts.

Conclusions: Our results suggest that expression of antigen TF and galectin-3 seems to participate in fibroadenoma development.

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Figures

Figure 1
Figure 1
Lectin and anti-galectin-3 histochemistry in healthy breast and fibroadenoma samples. Amaranthus leucocarpus recognizes ducts in healthy breast samples (A1): in fibroadenoma, Amaranthus leucocarpus lectin recognizes ducts and stroma cells (A2). Artocarpus integrifolia lectin recognizes ducts in healthy (B1) and fibroadenoma samples (B2). Arachis hypogaea recognizes ducts in healthy (C1) and in fibroadenoma samples (C2). Anti-galectin-3 antibody depicts weak staining in ducts of healthy samples (D1); in fibroadenoma, the antibody recognizes ducts and stroma cells (D2) Arrows indicate the lectin and antibody binding sites. Black arrow indicates ducts site of lectin or antibody binding. Yellow arrow indicates stroma site of lectin or antibody binding. Micrographs are in 10X.
Figure 2
Figure 2
Lectin and anti-galectin-3 double labeling histochemistry, in healthy breast and fibroadenoma samples. Weak staining with anti-galectin-3 was observed in healthy samples (A). Artocarpus integrifolia lectin recognizes ducts and stroma in healthy breast samples (B1). Arachis hypogaea recognizes ducts and stroma in healthy samples (C1). Amaranthus leucocarpus recognizes ducts and stroma in healthy breast samples (D1). In fibroadenoma, anti-galectin-3 recognizes ducts and stroma (B2) (C2) (D2). White arrow indicates ducts site of lectin or antibody binding. Yellow arrow indicates stroma site of lectin or antibody binding. Micrographs 10X.
Figure 3
Figure 3
Lectin and anti-galectin-3 double labeling histochemistry in fibroadenoma samples.Artocarpus integrifolia lectin recognizes luminal cells of ducts (A1). No interaction with luminal cells was observed in fibroadenoma using anti-galectin-3 (A2). White arrow indicates ducts site of lectin or antibody binding. Micrographs (40X).
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