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Comparative Study
. 2013 Feb 15;92(2):189-91.
doi: 10.1016/j.mimet.2012.12.006. Epub 2012 Dec 20.

Quantification of Candida albicans by flow cytometry using TO-PRO(®)-3 iodide as a single-stain viability dye

Monique Kerstens  1 , Gaëlle Boulet, Isabel Pintelon, Mario Hellings, Liesbeth Voeten, Peter Delputte, Louis Maes, Paul Cos
Affiliations
Comparative Study

Quantification of Candida albicans by flow cytometry using TO-PRO(®)-3 iodide as a single-stain viability dye

Monique Kerstens et al. J Microbiol Methods. .

Abstract

This study investigated a flow cytometric, single-stain approach to quantify Candida albicans as an alternative for the standard viable plate count. TO-PRO(®)-3 iodide allows an excellent distinction between viable and dead cells. Both quantification methods show a high degree of correlation.

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