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. 2012 Dec;13(4):377-83.
doi: 10.4142/jvs.2012.13.4.377.

A survey of porcine reproductive and respiratory syndrome among wild boar populations in Korea

Eun-Jin Choi  1 , Chang-Hee Lee, Bang-Hun Hyun, Jae-Jo Kim, Seong-In Lim, Jae-Young Song, Yeun-Kyung Shin
Affiliations

A survey of porcine reproductive and respiratory syndrome among wild boar populations in Korea

Eun-Jin Choi et al. J Vet Sci. 2012 Dec.

Abstract

No information is currently available on porcine reproductive and respiratory syndrome virus (PRRSV) infection in wild boars (Sus scrofa) in Korea. In this study, the status of PRRS in wild boars was investigated. Blood samples were collected from 267 wild boars from eight provinces in Korea. Four of the samples tested (1.5%) were positive for PRRSV antibodies and eight (3.0%) were positive for antigens. Of the virus-positive samples, three and five samples were typed as containing European (EU, type 1) or North American (NA, type 2) viruses, respectively. Two amplicons (one from type 1 and one from type 2) were used to analyze the PRRSV open reading frame 7 (ORF7) sequence. The nucleotide sequences of type 1 PRRSV ORF7 had identities between 96.1% and 98.4% with PRRSVs from domestic pigs in Korea. The sequences of type 2 PRRSV ORF7 had identities of 100% with the PRRSV strain VR-2332, which was prototypic North American strain. These results show that PRRSVs are present in wild boars in Korea, and effective PRRSV surveillance of the wild boar population might therefore be useful for disease control.

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Figures

Fig. 1
Fig. 1
RT-PCR results for the detection and differentiation of PRRSV in wild boar serum samples. Lane M: 100-bp DNA ladder, Lanes 1, 2, 3, 7, and 8: sample Nos. 49, 77, 167, 193, and 227, respectively (433-bp band), Lanes 4, 5, and 6: sample Nos. 110, 129, and 258, respectively (398-bp band), Lane 9: normal wild boar serum used as a negative control, Lane 10: VR-2332 strain used as a positive control for type 2, Lane 11: Lelystad virus (LV) used as a positive control for type 1.
Fig. 2
Fig. 2
Alignments of the putative amino acid sequences based on ORF7 of wild boar-derived PRRSVs and prototype virus strains. (A) No. 129 and Lelystad virus (LV). (B) No. 49 and VR-2332 strain. Dots indicate identical amino acids.
Fig. 3
Fig. 3
Phylogenetic analysis of ORF7 nucleotide sequences of a wild boar-derived and other various PRRSVs. The phylogenetic tree was constructed with wild boar type 1 (No. 129) PRRSV and PRRSV strains isolated worldwide. PRRSV strains are denoted as follows: PRRSV strain/GenBank accession No./country/collection time, publication year, or vaccine.
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