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. 2012 Dec;35(4):770-6.
doi: 10.1590/S1415-47572012005000077. Epub 2012 Nov 13.

Sex chromosome system ZZ/ZW in Apareiodon hasemani Eigenmann, 1916 (Characiformes, Parodontidae) and a derived chromosomal region

Elisangela Bellafronte  1 , Michelle Orane Schemberger, Roberto Ferreira Artoni, Orlando Moreira Filho, Marcelo Ricardo Vicari
Affiliations

Sex chromosome system ZZ/ZW in Apareiodon hasemani Eigenmann, 1916 (Characiformes, Parodontidae) and a derived chromosomal region

Elisangela Bellafronte et al. Genet Mol Biol. 2012 Dec.

Abstract

Parodontidae fish show few morphological characteristics for the identification of their representatives and chromosomal analyses have provided reliable features for determining the interrelationships in this family. In this study, the chromosomes of Apareiodon hasemani from the São Francisco River basin, Brazil, were analyzed and showed a karyotype with 2n = 54 meta/submetacentric chromosomes, and a ZZ/ZW sex chromosome system. The study revealed active NORs located on pair 11 and additional 18S rDNA sites on pairs 7 and 22. The 5S rDNA locus was found in pair 14. It showed a pericentric inversion regarding the ancestral condition. The satellite DNA pPh2004 was absent in the chromosomes of A. hasemani, a shared condition with most members of Apareiodon. The WAp probe was able to detect the amplification region of the W chromosome, corroborating the common origin of the system within Parodontidae. These chromosomal data corroborate an origin for the ZW system of Parodontidae and aid in the understanding of the differentiation of sex chromosome systems in Neotropical fishes.

Keywords: 18S rDNA; 5S rDNA; FISH; Neotropical fishes; cytogenetics.

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Figures

Figure 1
Figure 1
Karyotypes of males and female Apareiodon hasemani arranged from Giemsa-staining (a, c) and C-banding (b, d). Bar = 10 μm.
Figure 2
Figure 2
Chromosomal markers in Apareiodon hasemani (a) representative double-FISH karyotype, showing five 18S rDNA sites and chromosome pair 14 bearing 5S rDNA sites. (b) representative the WAp signals. The nucleolar organizer regions revealed by silver nitrate (Ag-NOR) staining are shown in the insert box. Bar = 10 μm.
Figure 3
Figure 3
Hypothetical derivation of the W chromosome from an ancestral chromosome similar to the Z chromosome in Apareiodon hasemani.
See this image and copyright information in PMC

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