[Isolation of DNA fraction bound to the axial structure of metaphase chromosomes and studies of renaturation]
[Article in
Russian]
- PMID: 232747
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[Isolation of DNA fraction bound to the axial structure of metaphase chromosomes and studies of renaturation]
[Article in
Russian]
Mol Biol (Mosk).
1979 Nov-Dec.
Abstract
Mitotic chromosomes of L cells (metaphase plates) were dehistonized by centrifugation through a layer of 2 M NaCl and then treated with restriction endonuclease EcoRI and HindIII. Alternatively, they were pretreated with EcoRI endonuclease. The DNA remaining attached to the axial structure of the chromosomes was isolated and investigated in renaturation experiments. It was found to be enriched in reiterated base sequences belonging to the satellite and to abundant intermediate repeats.
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