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. 1990 Feb 1;184(2):193-9.
doi: 10.1016/0003-2697(90)90668-y.

Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity

C R Wheeler  1 , J A Salzman, N M Elsayed, S T Omaye, D W Korte Jr
Affiliations

Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity

C R Wheeler et al. Anal Biochem. .

Abstract

Automated assays for catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase are presented. The assay for catalase is based on the peroxidatic activity of the enzyme. The glutathione peroxidase and reductase assays measure the consumption of NADPH following the reduction of t-butyl hydroperoxide and oxidized glutathione, respectively. The assay for superoxide dismutase is based on the reduction of cytochrome c. All assays utilize the Cobas FARA clinical automated analyzer and provide considerable time savings over the manual assays.

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