doi: 10.1016/j.jbiosc.2012.11.016.
Epub 2012 Dec 29.
Effects of hanging drop culture conditions on embryoid body formation and neuronal cell differentiation using mouse embryonic stem cells: optimization of culture conditions for the formation of well-controlled embryoid bodies
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- PMID: 23276518
- DOI: 10.1016/j.jbiosc.2012.11.016
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Effects of hanging drop culture conditions on embryoid body formation and neuronal cell differentiation using mouse embryonic stem cells: optimization of culture conditions for the formation of well-controlled embryoid bodies
J Biosci Bioeng.
2013 May.
Abstract
Hanging drop (HD) cultures were carried out with a drop volume of either 20 or 30 μl. An incubation period of 3 days was determined to be appropriate for the formation of well-controlled embryoid bodies (EBs), and the initial cell number was identified as the most critical factor in the growth and neuronal cell differentiation of EBs.
Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
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