Study of C-reactive protein and myocardial infarction in the Indian population

Abstract

To analyse the association of high sensitivity C-reactive (hsCRP) protein levels and -717A/G single nucleotide polymorphism of CRP with acute myocardial infarction (AMI) in the Indian population. Study population included 100 MI cases wherein 32 patients had experienced previous MI (MI-Group-1), 68 MI cases were recruited at presentation (MI-Group-2) and equal number of age and gender matched healthy individuals. hsCRP levels were determined by ELISA and genotyping of -717A/G was carried out by polymerase chain reaction-based restriction digestion method. The -717A/G genotypes did not influence hsCRP level and their distribution did not differ between groups. However, in the present study hsCRP demonstrated significant correlation with BMI in controls of both the genders and with triglycerides in females of AMI at presentation who otherwise are with low risk profile. Identifying traditional risk factors associated with inflammation may help in controlling the acute event.

Keywords: C-reactive protein; Indian population; Myocardial infarction; Single nucleotide polymorphism.

Fig. 1

Restriction digestion pattern of −717A/G polymorphism of CRP gene. Lane 1: O′ Range Ruler 20 bp DNA Ladder. Lane 2: Cut PCR product showing one band of 110 bp of homozygous mutant (G-717G). Lane 3: Uncut PCR product showing one band of 136 bp of homozygous wild type (A-717A). Lane 4: Partially cut PCR product showing two bands of 136 bp and 110 bp of heterozygous mutant (A-717G). Lane 5: Undigested PCR product of 136 bp

Fig. 2

Sequencing of −717A/G genotypes

Fig. 3

Risk stratified hsCRP levels amongst study groups

Fig. 4

hsCRP levels (mg/l) within group as per BMI (kg/m²)

Fig. 5

Distribution of hsCRP levels amongst the three study groups. 1 = Control group, 2 = MI-Group-1 and 3 = MI-Group-2

Fig. 6

hsCRP levels as per the three genotypes in all three study groups