MORFOREL, a computer program for two-dimensional analysis of micrographs of biological specimens, with emphasis on immunogold preparations

Abstract

The program is a tool for accelerating analysis of tissue components (profiles) as seen in micrographs, including electron micrographs with immunoreactive substances labelled with gold particles. Required equipment is a computer with digitizer and printer. From coordinates sampled around the profiles, area, perimeter and form factor are calculated; particles in profiles, when wanted, are counted to obtain particle densities. MORFOREL permits basic statistical calculations on primary data or on composite expressions based on them. Expressions can be saved on disk and retrieved. Primary and calculated data are readily output in a format readable by common commercial packages.