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. 2013 Jun;32(6):781-6.
doi: 10.1007/s10096-012-1808-2. Epub 2013 Jan 4.

Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH

C Almeida  1 , N F Azevedo, J C Bento, N Cerca, H Ramos, M J Vieira, C W Keevil
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Free article

Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH

C Almeida et al. Eur J Clin Microbiol Infect Dis. 2013 Jun.
Free article

Abstract

We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2-99.7) and 100 % (95 % CI, 80,8-100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.

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