Cyclic nature of poly(3-hydroxyalkanoate) metabolism in Alcaligenes eutrophus

Abstract

The cyclic process of biosynthesis and degradation of poly(3-hydroxyalkanoate) (PHA) was studied in Alcaligenes eutrophus under conditions of nitrogen-limitation of growth. A. eutrophus cells, which had accumulated poly(3-hydroxybutyrate) (PHB) of 55 wt% content within cells from butyric acid, were transferred into a nitrogen-free medium containing pentanoic acid as the sole carbon source and cultivated at 30 degrees C and pH 7.5. The content of PHB in A. eutrophus cells decreased with time, whereas a copolyester of 3-hydroxybutyrate (HB) and 3-hydroxyvalerate (HV) units, P(HB-co-HV), was accumulated in the presence of pentanoic acid. Conversely, when A. eutrophus cells with 50 wt% content of P(HB-co-56% HV) were incubated in a nitrogen-free medium containing butyric acid, the content of P(HB-co-56% HV) decreased with time, whereas PHB was accumulated. These results indicate the cyclic nature of PHA metabolism in A. eutrophus under these conditions.