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. 2012 Sep;19(3):245-9.
doi: 10.4103/0971-6580.103658.

Protective Effect of Psidium guajava in Arsenic-induced Oxidative Stress and Cytological Damage in Rats

Neeraj Tandon  1 , Manju Roy, Sushovan Roy, Neelu Gupta
Affiliations

Protective Effect of Psidium guajava in Arsenic-induced Oxidative Stress and Cytological Damage in Rats

Neeraj Tandon et al. Toxicol Int. 2012 Sep.

Abstract

This study was undertaken to evaluate the protective effect of aqueous extract of Psidium guajava leaves against sodium arsenite-induced toxicity in experimental rats. Animals were divided into four groups. Control group received arsenic free distilled water and three treatment groups (II, III, and IV) exposed to the arsenic (NaAsO(2)) (20 mg/kg b.wt) through drinking water. Group III and IV were administered a daily oral dose of P. guajava leaf extract 50 and 100 mg/kg b.wt. (AEPG(50) and AEPG(100)) for the period of 6 weeks. Blood samples and organs were collected at the end of the experiment. Arsenic exposure resulted in significant rise in lipid peroxidation (LPO) levels in erythrocyte, liver, kidney, and brain. In addition toxin decreased (P<0.05) the level of reduced glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) activities in the studied tissues. Residual effect of arsenic in various tissues was also observed. Histopathological results revealed mild to severe type of necrosis and degenerative changes in kidney and liver of arsenic intoxicated animals. Cytological alteration in brain tissue was also observed. Treatment with AEPG(100) (aqueous extract of P. guajava) @100 mg/kg body weight) significantly restored activities of oxidative stress markers like LPO levels, GSH levels, SOD, and CAT activities but having the limited protective activity of the herbal extract was observed on tissues architecture. It is therefore concluded that prophylactic co-administration of AEPG could provide specific protection from oxidative injury and to some extent on tissue damage.

Keywords: Histopahtology; Psidium guajava; oxidative stress; rats; sodium arsenite.

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Conflict of interest statement

Conflict of Interest: None declared.

References

    1. Ranft U, Miskovic P, Pesch B, Jakubis M, Nieuwenhuijsen MJ. EXPASCAN study group Association between arsenic exposure from a coal burning power plant and urinary arsenic concentrations in prievidza district. Slovakia. Environ Health Perspect. 2003;111:864–9. - PMC - PubMed
    1. Roy M, Pandey PK, Roy S, Chauhan HV. Arsenic induced haematobiochemical and histopathological alterations and its level in certain biological samples. Toxicol Int. 2008;25:57–62.
    1. Sarkar M, Chaudhuri GR, Chattopadhyay A, Biswas NM. Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats. Asian J Androl. 2003;5:27–31. - PubMed
    1. Ramos O, Carrizales L, Yáñez L, Mejía J, Batres L, Ortíz D, et al. Arsenic increased lipid peroxidation in rat tissues by a mechanism independent of glutathione levels. Environ Health Perspect. 1995;103:85–8. - PMC - PubMed
    1. Droge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002;82:47–95. - PubMed