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. 2012 Nov;51(6):815-9.

Duration of action of sustained-release buprenorphine in 2 strains of mice

Elizabeth T Carbone  1 , Krista E Lindstrom, Sandy Diep, Larry Carbone
Affiliations

Duration of action of sustained-release buprenorphine in 2 strains of mice

Elizabeth T Carbone et al. J Am Assoc Lab Anim Sci. 2012 Nov.

Abstract

Buprenorphine HCl is a common analgesic for laboratory mice undergoing surgical procedures. The documented duration of action of buprenorphine HCl is as short as 3 to 5 h in mice, potentially necessitating readministration for continued analgesia. A long-acting buprenorphine formulation would reduce handling-associated stress and provide uninterrupted analgesia. This study used the hot-plate assay to assess the antinociceptive effects of a single injection of sustained-release buprenorphine (bup-SR), buprenorphine-HCl (bup-HCl), and saline over 72 h in young adult male BALB/cJ and SWR/J mice. SWR/J mice had shorter baseline latencies than did BALB/cJ mice, possibly reflecting greater sensitivity to thermal nociception. Relative increase from baseline latency (% maximal possible effect) was significant for buprenorphine-SR at 2, 6, and 12 h compared with saline. According to results from a hot-plate assay, the analgesic efficacy of buprenorphine-SR appears to last at least 12 h in male BALB/cJ and SWR/J mice.

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Figures

Figure 1.
Figure 1.
Hot-plate apparatus and endpoint behavior. Two observers were present during data collection, and a digital camera and mirror were set up to allow subsequent review of video recordings. This mouse is licking his hind paw, a nocifensive behavior.
Figure 2.
Figure 2.
Percentage of maximal possible effect (%MPE; mean ± SEM, n = 15) for each treatment group at each time point. Significant differences were present at 2 (sustained-release buprenorphine [bup-SR] > buprenorphine-HCl [bup-HCl]; bup-SR > saline), 6 (bup-SR > saline), 12 (bup-SR > saline), and 72 (bup-SR > bup-HCl) h. *, Significant (P < 0.05) difference between bracketed values.
See this image and copyright information in PMC

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