Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Dec;107(8):1073-5.
doi: 10.1590/s0074-02762012000800020.

Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil

Vanessa Melandri  1 , Anthony Érico Guimarães, Nicholas Komar, Maurício L Nogueira, Adriano Mondini, Ana Fernandez-Sesma, Jeronimo Alencar, Irene Bosch
Affiliations

Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil

Vanessa Melandri et al. Mem Inst Oswaldo Cruz. 2012 Dec.

Abstract

In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.

PubMed Disclaimer

Figures

Figure
Figure
Location of sampling in the Pantanal ecological reserve, state of Mato Grosso, Brazil.
See this image and copyright information in PMC

References

    1. Beaty BJ, Calisher CH, Shope RE. Diagnostic procedures for viral, rickettsial and chlamydial infections. In: Lennette EH, Lennette DA, Lennette ET, editors. Arboviruses. Washington DC: American Public Health Association; 1995. pp. 189–212.
    1. Blitvich BJ, Bowen RA, Marlenee NL, Hall RA, Bunning ML, Beaty BJ. Epitope-blocking enzyme-linked immunosorbent assays for detection of West Nile virus antibodies in domestic mammals. J Clin Microbiol. 2003;41:2676–2679. - PMC - PubMed
    1. Bosch I, Herrera F, Navarro J, Lentino M, Dupuis A, Maffei J, Jones M, Fernández E, Pérez N, Pérez-Emán J, Guimarães AE, Barrera R, Valero N, Ruiz J, Velásquez G, Martinez J, Comach G, Komar N, Spielman A, Kramer L. West Nile virus, Venezuela. Emerg Infect Dis. 2007;13:651–653. - PMC - PubMed
    1. Diaz LA, Komar N, Visintin A, Júri MJD, Stein M, Allende RL, Spin-santi L, Konigheim B, Aguilar J, Laurito M, Almirón W, Contigiani M. West Nile virus in birds, Argentina. Emerg Infect Dis. 2008;14:689–691. - PMC - PubMed
    1. Komar N, Clark GG. West Nile virus activity in Latin America and Caribbean. Rev Panam Salud Publica. 2006;19:112–117. - PubMed

Publication types

Substances