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. 1990 Mar;31(3):215-23.
doi: 10.1016/0165-0270(90)90113-t.

Design of a microprocessor-controlled thermoregulated chamber for perfusing in vitro mammalian brain preparations

M Mühlethaler  1 , P Cand, R Henauer, M Serafin
Affiliations

Design of a microprocessor-controlled thermoregulated chamber for perfusing in vitro mammalian brain preparations

M Mühlethaler et al. J Neurosci Methods. 1990 Mar.

Abstract

A system is described that allows to perfuse in vitro isolated mammalian brain preparations obtained from adult guinea-pigs. It consists of a microprocessor controlled thermoregulation unit, a perfusion line and a perfusion/recording chamber. The main features of the thermoregulation unit include the ability to maintain stable temperatures between 10 and 37 degrees C and to precisely control the time course of the temperature changes. Using this system, perfused tissue blocks can be kept viable for 8-10 h.

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