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. 2013 Feb 15;288(7):5062-71.
doi: 10.1074/jbc.X113.451591. Epub 2013 Jan 8.

Practicing biochemistry without a license

H Franklin Bunn  1
Affiliations

Practicing biochemistry without a license

H Franklin Bunn. J Biol Chem. .
No abstract available

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Figures

FIGURE 1.
FIGURE 1.
Max Perutz (left) and H. F. B.
SCHEME 1.
SCHEME 1.
Biosynthesis of Hb AIc.
FIGURE 2.
FIGURE 2.
Specific radioactivity of the major hemoglobin component (Hb A) and Hb AIc after injection of 59Fe-labeled transferrin into a normal compliant “volunteer.”
FIGURE 3.
FIGURE 3.
A, effect of charge on the proportion of abnormal hemoglobin in individuals heterozygous for 72 stable β-globin variants. Each data point represents a mean value for a given variant. B, effect of α-thalassemia on the proportion of seven positively charged β subunit variants (blue) and three negatively charged variants (red).
FIGURE 4.
FIGURE 4.
H. F. B. with Bill Eaton (right) in Israel.
FIGURE 5.
FIGURE 5.
Impact of oxygenation on HIF-1α stability. EMSAs and Western blotting were carried out on extracts of oxygenated HeLa cells (N) and on cells incubated in 1% O2 for 4 h and then, at time 0, exposed to 21% O2. HIF-1α protein remained stable, whereas HIF-1α protein decayed rapidly. H, hypoxia; N, normoxia.
FIGURE 6.
FIGURE 6.
Layout of Ncb5or protein. The cytochrome b5 (cyt b5) domain at the N terminus contains two highly conserved histidines that bind heme iron (H). The cytochrome b5 reductase (cyt b5R) domain at the C terminus contains flavin (FAD)- and NADPH-binding domains.
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